The Biological bulletin (20187998518)

Title: The Biological bulletin
Identifier: biologicalbullet126mari (find matches)
Year: [1] (s)
Authors: Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983
Subjects: Biology; Zoology; Biology; Marine Biology
Publisher: Woods Hole, Mass. : Marine Biological Laboratory
Contributing Library: MBLWHOI Library
Digitizing Sponsor: MBLWHOI Library

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230 i K \\K M. RSI IKK. JJ<. r.ffcct of sitbsfruti' t'oncciili'dtioii and inhibition Tlu- effect of cellobiose concentration T.S-. enzymatic activity was examined using nine substrate ntrations over a 100-fold range. The A",,, was estimated to be 5.. 10- /-'.moles/ing, protein hour from the double I/S
Text Appearing After Image:
5 10 20 ' 40 jj MOLES CELLOBIOSE FIGTKK 5. Substrate concentration plot: (1) A-A specific activity 7'.v. concentration of .•rllobiosc ; (2) • • 1/7- vs. \/s. Conditions: 60 /umoles buffer (phtlialate pH 5.0). HOH+ Mib.-itratc to equal 1.7 ml., 0.1 nil. en/.yme ( 60rv acetone fraction, animal gut). TABLE VII 7\ ,„ and A,,,,,.r vnhtes n^ninsl rtirioHx glucosides. Conditions: 20 /j,moles substrate, f>0 nmoles Intfirr (/i/itluild tc /)H 5.0), O.I ml. enzyme (h()' , at clone fnutinu, mutual »///) ^ul>-i rate Inhibitor KmXlO" Alii'iirt-nt K,,, X10:l Vmax /c,xio:) p-nitrophenol ;8-D-glucoside — 16.56 62.5 — Lactose 2.5X10 - 56. 1 7 55.6 9.0 Cellobiose 2.5X10 ' M 126.40 58.8 3.0 Glucose 1.25X10 - .17 65.07 58.1 4.0 o-nitrophenol-/3-) )-;idc 23.2 14.3 Arbutin 1.2 11.7 Salicin 0.621 17.2 ( 'cllol 0.563 188.6

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